Supplementary Materials

The PDF file includes:

  • Fig. S1. Systemic [Ca2+]cyt waves and SWPs induced by cutting a lateral root.
  • Fig. S2. Leaf-to-leaf SWPs induced by wounding and glutamate.
  • Fig. S3. Participation of GLR3.3 and GLR3.6 in wound- and glutamate-triggered root-to-shoot [Ca2+]cyt waves.
  • Fig. S4. Inhibition of glutamate-induced SWPs by FC.
  • Fig. S5. Conductivity of GLR3.3 to divalent and monovalent cations.
  • Fig. S6. Conductivity of GLR3.6 to divalent and monovalent cations.
  • Table S1. Relative ion permeability of GLR3.3 and GLR3.6.
  • Table S2. Primers.
  • Legends for movies S1 to S7

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Systemic [Ca2+]cyt waves in response to cutting the main root.
  • Movie S2 (.avi format). Root-to-shoot [Ca2+]cyt waves induced by application of glutamate at the wound site.
  • Movie S3 (.avi format). Loss of root-to-shoot [Ca2+]cyt waves in a glr3.3glr3.6 mutant upon root wounding.
  • Movie S4 (.avi format). Loss of root-to-shoot [Ca2+]cyt waves in a glr3.3glr3.6 mutant upon application of glutamate at the wound site.
  • Movie S5 (.avi format). [Ca2+]cyt imaging of control HEK293T cells upon application of glutamate at pH 8.5.
  • Movie S6 (.avi format). [Ca2+]cyt imaging of GLR3.6-expressing HEK293T cells upon application of glutamate at pH 8.5.
  • Movie S7 (.avi format). [Ca2+]cyt imaging of GLR3.6-expressing HEK293T cells upon application of histidine at pH 8.5.