Supplementary Materials

The PDF file includes:

  • Fig. S1. Data quality of the 1-hour peptidomics approach in analysis of hypothalamus peptides.
  • Fig. S2. Quality control of the quantitative peptidomics.
  • Fig. S3. Gut microbiome compositions resulting from 16S rRNA analysis.
  • Fig. S4. Histogram illustrates the total quantifiable and significantly altered hypothalamus peptides (unique sequences) of the CC/C dataset resulting from 1-month development of mice.
  • Fig. S5. Hierarchical clustering shows the brain peptide change in hypothalamus among the C, NS8, and NS9 groups.
  • Table S1. Neuropeptide families.
  • Legends for data files S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Identified peptides from four brain regions (corresponding to Fig. 2A) (Excel file).
  • Data file S2. Detailed information of peptides shown in Fig. 2D (Excel file).
  • Data file S3. Detailed information of correlation analysis shown in Fig. 3C (Excel file).
  • Data file S4. Detailed information of clustering analysis shown in Fig. 3E (Excel file).
  • Data file S5. Detailed information of peptidome shown in Fig. 4A (Excel file).
  • Data file S6. Detailed information of peptidome shown in Fig. 4D (Excel file).
  • Data file S7. Detailed information of peptidome shown in Fig. 5, D and F (Excel file).
  • Data file S8. Percentage of basic-cleavage-siteā€“containing peptides (Excel file).
  • Data file S9. Detailed information of MINIbar script (Excel file).