Supplementary Materials

The PDF file includes:

  • Fig. S1. Clone 4 CTLs efficiently kill KdHA-pulsed splenocytes in vivo.
  • Fig. S2. Diminished MTOC polarization of clone 4 TILs toward the T cell–target cell interface.
  • Fig. S3. F-actin distribution is impaired in TILs.
  • Fig. S4. Greater enrichment of cofilin, coronin 1A, and chronophin in TILs than in CTLs.
  • Fig. S5. Increased cofilin phosphorylation but reduced cofilin abundance in TILs.
  • Fig. S6. Characterization of the RencaHA PD-L1−/− cell line and corresponding tumors.
  • Fig. S7. Loss of PD-1 engagement in vivo rescues cytoskeletal polarization.
  • Fig. S8. Acute in vitro blockade of PD-1 does not restore cytoskeletal polarization.
  • Fig. S9. Data pooling.
  • Legends for movies S1 to S7

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Representative interactions of a tubulin-GFP–expressing clone 4 CTL and TIL with Renca+HA target cells.
  • Movie S2 (.mp4 format). Representative interactions of an F-tractin–GFP–expressing clone 4 CTL, TIL, and Jasplakinolide-treated CTL with Renca+HA target cells.
  • Movie S3 (.mp4 format). Representative interactions of a cofilin-GFP–expressing clone 4 CTL and TIL with Renca+HA target cells.
  • Movie S4 (.mp4 format). Representative interaction of a coronin 1A–GFP–expressing clone 4 CTL with a Renca+HA target cell.
  • Movie S5 (.mp4 format). Representative interactions of an Arp3-GFP–expressing clone 4 CTL and TIL with Renca+HA target cells.
  • Movie S6 (.mp4 format). Representative interaction of a chronophin-GFP–expressing clone 4 CTL with a Renca+HA target cell.
  • Movie S7 (.mp4 format). Representative interactions of a Fura-2–loaded clone 4 CTL and TIL with a Renca+HA target cell.