Supplementary Materials

The PDF file includes:

  • Fig. S1. Consistency among haploid genetic screens for p-RPS6 obtained with different sorting settings.
  • Fig. S2. Analysis of p-RPS6 regulators.
  • Fig. S3. Rapamycin dose-response curve using p-RPS6 as the readout.
  • Fig. S4. Modulation of p-RPS6 levels in ΔLAMTOR4 and ΔLAMTOR5 cells in the presence of rapamycin.
  • Fig. S5. Estimation of FLCN genetic interactions.
  • Fig. S6. Depletion of ΔLAMTOR4ΔFLCN cells from gene-edited cell pools.
  • Fig. S7. Mechanism of FLCN up-regulation in ΔLAMTOR4 and ΔLAMTOR5 cells.
  • Table S1. List of plasmids used in this study.
  • Table S2. List of primers used to recover insertion sites in FACS-based genetic screen.
  • Table S3. List of sgRNAs used in this study.
  • Table S4. List of primers used in this study.
  • Legends for data files S1 to S8
  • References (6163)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in WT HAP1 cells (location, NKI; sorting gates, 1%).
  • Data file S2 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in WT HAP1 cells (location, OX; sorting gates, 1%).
  • Data file S3 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in WT HAP1 cells (location, OX; sorting gates, 3%).
  • Data file S4 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in WT HAP1 cells (combined data from OX screens with 1 and 3% sorting gates).
  • Data file S5 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in rapamycin-treated WT HAP1 cells.
  • Data file S6 (Microsoft Excel format). List of ΔMI values in haploid genetic screens comparing mTORC1 activation in the absence and presence of rapamycin.
  • Data file S7 (Microsoft Excel format). Haploid genetic screen for mTORC1 activation in ΔLAMTOR4 HAP1 cells.
  • Data file S8 (Microsoft Excel format). List of positive regulators specific for the genetic screen performed in ΔLAMTOR4 HAP1 cells.