Supplementary Materials

The PDF file includes:

  • Fig. S1. Yeast surface display selection for high-affinity monomeric IL-10 variants and recombinant expression of WT and high-affinity IL-10 monomeric and dimeric variants.
  • Fig. S2. Biophysical characterization of the high-affinity IL-10 variants.
  • Fig. S3. Single-molecule imaging of IL-10 receptors by TIRF microscopy.
  • Fig. S4. Extended kinetics of IL-10 and variants in human monocytes.
  • Fig. S5. Analysis of gene expression profiles induced by WT IL-10 and high-affinity variants in human monocytes.
  • Fig. S6. Characterization of the IL-10–treated CD8+ T cell phenotype.
  • Fig. S7. Analysis of gene expression profiles induced by WT IL-10 and high-affinity variants in human CD8+ T cells.
  • Legends for data files S1 and S2

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). RNA sequencing dataset for monocytes.
  • Data file S2 (Microsoft Excel format). RNA sequencing dataset for CD8+ T cells.