Supplementary Materials

The PDF file includes:

• Fig. S1. Yeast surface display selection for high-affinity monomeric IL-10 variants and recombinant expression of WT and high-affinity IL-10 monomeric and dimeric variants.
• Fig. S2. Biophysical characterization of the high-affinity IL-10 variants.
• Fig. S3. Single-molecule imaging of IL-10 receptors by TIRF microscopy.
• Fig. S4. Extended kinetics of IL-10 and variants in human monocytes.
• Fig. S5. Analysis of gene expression profiles induced by WT IL-10 and high-affinity variants in human monocytes.
• Fig. S6. Characterization of the IL-10–treated CD8⁺ T cell phenotype.
• Fig. S7. Analysis of gene expression profiles induced by WT IL-10 and high-affinity variants in human CD8⁺ T cells.
• Legends for data files S1 and S2

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). RNA sequencing dataset for monocytes.
• Data file S2 (Microsoft Excel format). RNA sequencing dataset for CD8⁺ T cells.