Supplementary Materials

The PDF file includes:

  • Fig. S1. Macrophage polarization.
  • Fig. S2. Macrophages drive changes in metabolism and metabolic gene expression in breast cancer cells.
  • Fig. S3. STAT and TGF-β pathways mediate macrophage-dependent SDHD expression changes.
  • Fig. S4. AI macrophages stabilize HIF1α levels and transcriptional activity.
  • Fig. S5. TAMs regulate tumor metabolism in vivo.
  • Fig. S6. Ex vivo polarized macrophages are recruited to the tumor site in an orthotopic model.
  • Fig. S7. Ex vivo polarized macrophages regulate in vivo tumor metabolism, vascularization, and immune evasion.
  • Table S1. List of antibodies used in flow cytometry experiments for macrophage characterization.
  • Legend for data file S1
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1. Excel file containing microarray KEGG analysis and individual gene list.
  • Movie S1 (.mp4 format). HIF1α activation in MDA-MB-231 cancer cells cocultured with PI macrophages.
  • Movie S2 (.mp4 format). HIF1α activation in MDA-MB-231 cancer cells cocultured with AI macrophages.