Supplementary Materials

The PDF file includes:

• Fig. S1. BfmR-responsive reporter activity in MPAO1 and bfmS-DK2 cells under various growth conditions.
• Fig. S2. Consequences of bfmS missense mutations or bfmR deletion.
• Fig. S3. Identification of genes involved in activating BfmR.
• Fig. S4. Glucose increases the transcriptional regulatory activity of GtrS.
• Fig. S5. Phosphorylation of BfmR by GtrS and coimmunoprecipitation of BfmR and GtrS.
• Fig. S6. Bacterial two-hybrid assays showing GtrS-BfmR interactions.
• Fig. S7. Sequence alignment of the histidine kinases BfmS, GtrS, EnvZ, and HK853.
• Fig. S8. Promoter fusion analysis and phosphorylation assays.
• Fig. S9. Pro-Q Diamond staining and SPR assays.
• Fig. S10. Role of natural bfmS variants in BfmR activation.
• Fig. S11. Dephosphorylation of BfmR by wild-type BfmSc and its variants.
• Table S1. Plasmids and bacterial strains and used in this study.
• Table S2. Primers used in this study.
• Table S3. bfmS variants in P. aeruginosa CF isolates.
• Legends for data files S1 and S2
• References (67–82)

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Microarray data analysis of DK2.
• Data file S2 (Microsoft Excel format). RNA-seq analysis.