Supplementary Materials

This PDF file includes:

  • Fig. S1. DKK1-overexpressing and DKK1-knockdown Huh-7 cells.
  • Fig. S2. EGFR activation promotes DKK1 expression in HCC through the MEK-ERK and PI3K-Akt pathways.
  • Fig. S3. ERK1/2-dependent phosphorylation and nuclear translocation of PKM2 promote DKK1 transcription in HepG2 cells.
  • Fig. S4. Akt-dependent p300 phosphorylation mediates the EGF-induced increase in DKK1 transcription in HepG2 cells.
  • Fig. S5. Cotransfection of the PKM2 S37D mutant with the p300 S1834D mutant produced a synergistic effect on DKK1 expression in HepG2 cells.
  • Fig. S6. PKM2-dependent phosphorylation of histone H3 at Thr11 mediates EGFR-induced histone H3-Lys9 acetylation and DKK1 transcription in HepG2 cells.
  • Fig. S7. p300-dependent acetylation of histone H3 mediates EGFR-induced DKK1 transcription in HepG2 cells.

[Download PDF]