Supplementary Materials

The PDF file includes:

  • Fig. S1. The regulatory network for metabolic reactions.
  • Fig. S2. Oral glucose administration and multiomics measurements.
  • Fig. S3. Differences in the amounts of molecules between WT mice and ob/ob mice before oral glucose administration.
  • Fig. S4. Time courses of lipidomic changes in the liver.
  • Fig. S5. Time courses of metabolite changes in the blood.
  • Fig. S6. Changes in the expression of glucose-responsive genes during oral water administration.
  • Fig. S7. Hierarchical clustering of the time courses of gene expression in the liver and inference of regulatory connections between transcription factors and genes.
  • Fig. S8. Western blotting for insulin signaling molecules.
  • Fig. S9. The regulatory transomics network for glucose-responsive metabolic reactions in glycogen metabolism.
  • Fig. S10. The regulatory transomics network for glucose-responsive metabolic reactions in glycolysis and gluconeogenesis.
  • Fig. S11. The regulatory transomics network for glucose-responsive metabolic reactions in lipid synthesis.
  • Fig. S12. The regulatory transomics network for glucose-responsive metabolic reactions in cholesterol synthesis.
  • Fig. S13. Km and Ki values of metabolic reactions regulated by ATP and NADP+.
  • Fig. S14. Metabolic reactions regulated by glucose-responsive molecules in each metabolic pathway node.
  • Fig. S15. T1/2 values of the interlayer regulatory connections from glucose-responsive molecules to metabolic reactions.
  • Legends for data files S1 to S15

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Metabolomic data in the liver.
  • Data file S2 (Microsoft Excel format). Lipidomic data.
  • Data file S3 (Microsoft Excel format). Metabolomic data in the blood.
  • Data file S4 (Microsoft Excel format). Transcriptomic data.
  • Data file S5 (Microsoft Excel format). Pathway enrichment analysis of glucose-responsive genes and genes showing the differences in the amounts of expression between WT mice and ob/ob mice before oral glucose administration.
  • Data file S6 (Microsoft Excel format). RT-PCR data.
  • Data file S7 (Microsoft Excel format). Enrichment analysis of gene clusters.
  • Data file S8 (Microsoft Excel format). Inferred regulatory connections between transcription factors and genes.
  • Data file S9 (Microsoft Excel format). Overlap between the inferred genes of transcription factors and those predicted from experimental ChIP data.
  • Data file S10 (Microsoft Excel format). Western blotting data.
  • Data file S11 (Microsoft Excel format). Regulatory transomics network for glucose-responsive metabolic reactions.
  • Data file S12 (Microsoft Excel format). Enrichment analysis of downstream genes of each transcription factor.
  • Data file S13 (Microsoft Excel format). Enzyme binding affinity Km and Ki.
  • Data file S14 (Microsoft Excel format). Significant associations between glucose-responsive molecules and metabolic pathways.
  • Data file S15 (Microsoft Excel format). Differences between WT mice and ob/ob mice, and differences between normal chow diet–fed mice and high-fat diet–fed mice.