Supplementary Materials

This PDF file includes:

  • Fig. S1. Protein constructs.
  • Fig. S2. Stereo diagrams of the CBP-E1963L structures.
  • Fig. S3. Stereo diagrams of the SMAD2-CBP complex structures.
  • Fig. S4. Structure of SMAD2 in complex with wild-type CBP.
  • Fig. S5. Amino acid sequence alignments.
  • Fig. S6. Interaction between SMAD2 and CBP.
  • Fig. S7. Stereo diagram of the MH2 domain of SMAD2.
  • Fig. S8. CBP and SKI recognition by SMAD2.
  • Fig. S9. ITC experiments with the CBP and SMAD1 mutants.
  • Fig. S10. Model structure of the SMAD2-SMAD2-SMAD4-FOXH1-CBP complex.
  • Fig. S11. Subcellular localization of CBP peptides in HepG2 cells.
  • Table S1. Summary of ITC of the interaction of CBP (His-GST-CBP) with SMAD2LC-2E.
  • Table S2. Summary of ITC of the interaction of CBP [His-GST-CBP(1941–1992)] with SMAD2LC-2E, SMAD1LC-2E, and its mutants.
  • Table S3. Synthesized DNA sequence of CBP.
  • Table S4. Primers used in this study.
  • Table S5. Summary of data collection and refinement statistics of the SMAD2-CBP complexes.

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