Supplementary Materials

The PDF file includes:

  • Fig. S1. Representative sensorgrams from SPR analysis of the interaction of Tlp10 LBD with various ligands.
  • Fig. S2. Mutant Tlp10LBD proteins are correctly folded.
  • Fig. S3. Invasion of mammalian cells by WT and mutant C. jejuni in the presence and absence of fucose and galactose.
  • Table S1. HHpred search results for small subset of DAHL domains from various species.
  • Table S2. Molecules included in the glycan microarray.
  • Table S3. Molecules included in the small-molecule microarray.
  • Table S4. Complex glycan structures recognized by Tlp10.
  • Table S5. Chemotaxis responses of WT and mutant C. jejuni strains.
  • Table S6. Illustration of A-B-A competition SPR analysis of WT Tlp10LBD.
  • Table S7. Illustration of A-B-A competition SPR analysis of Tlp10LBDY70A.
  • Table S8. Illustration of A-B-A competition SPR analysis of Tlp10LBDY115A.
  • Table S9. Illustration of A-B-A competition SPR analysis of Tlp10LBDY120A.
  • Table S10. Illustration of A-B-A competition SPR analysis of Tlp10LBDH193A.
  • Table S11. Analysis of protein-protein interactions with Tlp10sig using the yeast two-hybrid system.
  • Table S12. Fusion proteins for yeast two-hybrid experiments.
  • Table S13. Microbial strains and plasmids used in this study.
  • Table S14. DNA primers used in this study.
  • Table S15. MIRAGE glycan and small-molecule arrays for Tlp10.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). C. jejuni migrate toward the attractant isoleucine.
  • Movie S2 (.avi format). Migration of C. jejuni cells toward the attractant isoleucine is reduced by addition of the repellent arginine.
  • Movie S3 (.avi format). PBS control for the effect of arginine on the migration of WT C. jejuni toward isoleucine.
  • Data file S1 (Microsoft Excel format). Proteins included in DAHL domain MSA.