Supplementary Materials

The PDF file includes:

  • Fig. S1. Cytokine production by T cells in response to constant stimulation by the supraphysiological affinity pMHC 9V.
  • Fig. S2. Cytokine production by T cells in response to constant stimulation by the physiological affinity pMHC 4A8K.
  • Fig. S3. T cell adaptation is the result of reduced cytokine production per cell.
  • Fig. S4. Quantitative assessment of TCR down-regulation is independent of the detection method used.
  • Fig. S5. Posterior distributions of 3000 particles after 30 populations using ABC-SMC.
  • Fig. S6. T cells adapted to a low-affinity antigen can be reactivated with a higher-affinity antigen.
  • Fig. S7. T cells adapted to constant antigen can be reactivated with 4-1BB costimulation.
  • Fig. S8. Costimulation by CD28 and 4-1BB does not increase TCR surface expression after adaptation.
  • Fig. S9. CD28 costimulation does not prevent adaptation to constant antigen stimulation.
  • Fig. S10. Expression levels and antigen-induced down-regulation of first- and second-generation CARs.

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