Supplementary Materials
The PDF file includes:
- Materials and Methods
- Fig. S1. TNF linear increase with various ramping rates.
- Fig. S2. TNF exponential increase with various growth rates.
- Fig. S3. Additional IL-1β ramping and random fluctuation experiments.
- Fig. S4. Parameter sensitivity test for differentiator behavior in NF-κB system.
- Fig. S5. Fitting without A20 or the IKK module.
- Fig. S6. Production of an A20 knockout cell line.
- Fig. S7. Simulation for IκBα and A20.
- Fig. S8. Clustering single-cell traces in TNF and IL-1β exponential rampings.
- Fig. S9. Dose differentiation by NF-κB is sensitive to the strength of the negative feedback.
- Fig. S10. Clustering of single-cell traces shows a fundamental difference in the NF-κB response to Pam stimulus.
- Fig. S11. NF-κB simulation for Pam stimulation.
- Table S1. TNF dose and critical points used for fitting.
- Table S2. Parameters used for the simulation.
- Table S3. List of all custom designed primers and probes used for qPCR.
- References (64–72)
Other Supplementary Material for this manuscript includes the following:
