Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. TNF linear increase with various ramping rates.
  • Fig. S2. TNF exponential increase with various growth rates.
  • Fig. S3. Additional IL-1β ramping and random fluctuation experiments.
  • Fig. S4. Parameter sensitivity test for differentiator behavior in NF-κB system.
  • Fig. S5. Fitting without A20 or the IKK module.
  • Fig. S6. Production of an A20 knockout cell line.
  • Fig. S7. Simulation for IκBα and A20.
  • Fig. S8. Clustering single-cell traces in TNF and IL-1β exponential rampings.
  • Fig. S9. Dose differentiation by NF-κB is sensitive to the strength of the negative feedback.
  • Fig. S10. Clustering of single-cell traces shows a fundamental difference in the NF-κB response to Pam stimulus.
  • Fig. S11. NF-κB simulation for Pam stimulation.
  • Table S1. TNF dose and critical points used for fitting.
  • Table S2. Parameters used for the simulation.
  • Table S3. List of all custom designed primers and probes used for qPCR.
  • References (6472)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Cell images for TNF dose increase (ramp) experiments.
  • Movie S2 (.avi format). Cell images for IL-1β dose increase (ramp) experiments.