Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. Cell-cycle dependence of the transcriptional response.
  • Fig. S2. Quantifying persistence in response to a 90-min pulse of stimulus.
  • Fig. S3. Simple delay model.
  • Fig. S4. Score distribution for varied Hill coefficients.
  • Fig. S5. MAPK activation dynamics.
  • Fig. S6. PSTE5-STE12 and WT strains have similar basal Ste12 abundance.
  • Fig. S7. Degradation of Ste12 in the Ste12-Dig complex prevents adaptation.
  • Fig. S8. The model captures responses to dynamic stimulation.
  • Fig. S9. Parameter distributions.
  • Fig. S10. Effect of parameter distributions on the predicted response for the dig1Δdig2Δ strain.
  • Fig. S11. Persistence of different pathway perturbations in response to a 90-min pulse of stimulus.
  • Table S1. Plasmids used in this study.
  • Table S2. Oligonucleotide sequences.
  • Table S3. Strains used in this study.
  • Table S4. Parameter ranges for the evolutionary algorithm.
  • References (6181)

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