Supplementary Materials

The PDF file includes:

• Fig. S1. Endothelial-specific deletion of Drosha impairs erythropoiesis.
• Fig. S2. Knockdown of Drosha in K562 cells affects cell proliferation rate and impairs erythroid maturation.
• Fig. S3. Transcriptome analysis of EPCs from Ctrl and Drosha cKO embryos.
• Fig. S4. Quantitative proteomic analysis of RPs in K562 cells.
• Fig. S5. Association of Drosha and Dgcr8 at RPG loci.
• Fig. S6. Drosha and Dgcr8 preferentially bind to the 5′ end of RPG mRNAs.
• Fig. S7. Most RPG loci whose expression depends on Drosha contain R-loops.
• Fig. S8. Transcription initiation of RPGs is not affected in Drosha KO cells.
• Fig. S9. Immunoblot analysis of Drosha and Ddx5 in HCT116 cells expressing WT or HD Ddx5.
• Fig. S10. Reduction of global protein synthesis upon serum starvation.
• Fig. S11. Nedd4L does not play a role in the degradation of Drosha.
• Fig. S12. Nedd4 mRNA amounts are not increased by serum starvation.
• Table S1. Previously published high-throughput RNA-seq, ChIP-seq, eCLIP-seq, and DRIP-seq datasets analyzed in this paper.
• Table S2. Primers for mouse genotyping, qPCR, ChIP, and DRIP analysis.
• Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

• Data file S1 (Microsoft Excel format). Proteomics data.