Supplementary Materials

The PDF file includes:

  • Fig. S1. Validation of the InsPEx bioswitch method.
  • Fig. S2. Community analysis of the GO terms enriched in Cys ox-PTMs.
  • Fig. S3. Complete map of all enriched KEGG pathways.
  • Fig. S4. MS spectra of STING Cys oxidation sites under basal conditions and upon stimulation with HOCl.
  • Fig. S5. Quantification of protein phosphorylation as assessed by Western blotting.
  • Fig. S6. STING aggregates formed in response to 2′3′-cGAMP are sensitive to reducing agents.
  • Fig. S7. MS-based identification of STING Cys oxidation sites after stimulation with 2′3′-cGAMP.
  • Fig. S8. STING forms intramolecular disulfide bonds independently of Cys148 and Cys206.
  • Legends for data files S1 to S6
  • References (119132)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Mal-PEG2-Bio–modified peptides found in the pilot in-gel trypsinization approach in THP1 cells upon diamide treatment.
  • Data file S2 (Microsoft Excel format). Mal-PEG2-Bio–modified peptides found using the InsPEx approach in U937 cells upon diamide treatment.
  • Data file S3 (Microsoft Excel format). Biological triplicates of Mal-PEG2-Bio–modified peptides found with the InsPEx bioswitch approach under basal conditions and upon stimulation with diamide or HOCl.
  • Data file S4 (Microsoft Excel format). List of all Cys ox-PTM sites identified under basal- or oxidant-stimulated conditions.
  • Data file S5 (Microsoft Excel format). List of all GO terms that were enriched in Cys ox-PTMs under basal conditions and upon stimulation with diamide or HOCl.
  • Data file S6 (Microsoft Excel format). List of all KEGG pathways found enriched in oxidized proteins under basal conditions and upon stimulation with diamide or HOCl.