Supplementary Materials

The PDF file includes:

  • Fig. S1. Gating strategy used for cell sorting of human DC subpopulations.
  • Fig. S2. TLR expression and responsiveness of human DC subpopulations.
  • Fig. S3. Microarray analysis reveals tissue-independent superior expression of inflammasome-related molecules in human cDC2 under steady-state conditions.
  • Fig. S4. Priming with R848 induces strong transcriptional changes in cDC1 and cDC2 but does not change the identity of the cells.
  • Fig. S5. The CD1c+ DC subsets cDC2 and DC3 harbor a comparable expression of inflammasome genes.
  • Fig. S6. Comparable costimulatory molecule expression and morphological characteristics of control and L18-MDP–treated cDC2.
  • Fig. S7. ATP induces IL-1β secretion from pyroptotic cDC2 independently of the ATP concentration.
  • Fig. S8. Mixed leukocyte reaction shows similar, but weaker, T cell polarization profiles.
  • Fig. S9. Gating strategy for the restimulation of antigen-specific T cells with hyperactive cDC2.

[Download PDF]