Supplementary Materials

The PDF file includes:

  • Fig. S1. Biophysical characterization of P-Rex2.
  • Fig. S2. Biophysical characterization of the PTEN:P-Rex2 complex.
  • Fig. S3. Western blotting analysis of P-Rex2 and PTEN expression.
  • Fig. S4. EGF-dependent stimulation of Rac1 activity in transfected cells expressing P-Rex2 and PTEN (C124S) occurs through Gβγ subunits.
  • Fig. S5. Characterization of the PTEN:P-Rex2 interaction.
  • Fig. S6. Characterization of P-Rex2 PDZ2 cancer–associated somatic mutants.
  • Fig. S7. Characterization of truncated P-Rex2 somatic cancer–associated mutants.
  • Table S1. SPR analysis of the binding of P-Rex2 variants to PTEN.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following: