RT Journal Article
SR Electronic
T1 Reelin Multimers
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP tw3
OP tw3
DO 10.1126/stke.2000.46.tw3
VO 2000
IS 46
YR 2000
UL http://stke.sciencemag.org/content/2000/46/tw3.abstract
AB Reelin is a secreted protein that regulates neuron localization during development of the mammalian brain. Utsunomiya-Tate et al. analyzed neuronal cell lysates and culture media and found that Reelin forms large protein complexes of various sizes. An antibody known to bind to the amino-terminus of Reelin and to inhibit its biological activity also inhibited formation of Reelin multimers. Isolated epitope fragments of Reelin formed a linear, soluble polymer whose interaction is electrostatic in nature. A mutant form of Reelin lacking the epitope region did not form polymers nor could it transduce signals through Reelin receptors. The authors propose that the Reelin multimer may induce receptor oligomerization, although the size of the biologically active multimer is not yet known. Utsunomiya-Tate, N., Kubo, K., Tate, S., Kainosho, M., Katayama, E., Nakajima, K., and Mikoshiba, K. (2000) Reelin molecules assemble together to form a large protein complex, which is inhibited by the function-blocking CR-50 antibody. Proc. Natl. Acad. Sci. U.S.A. 97: 9729-9734. [Abstract] [Full Text]