RT Journal Article
SR Electronic
T1 Receptors Follow a Party Line
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP tw5
OP tw5
DO 10.1126/stke.2000.60.tw5
VO 2000
IS 60
YR 2000
UL http://stke.sciencemag.org/content/2000/60/tw5.abstract
AB A receptor is normally triggered when, and only when, its ligand binds to the receptor. It's not so simple for the ErbB1 receptor, which responds to stimulation with epidermal growth factor (EGF) by dimerization, cross phosphorylation of the monomers, and triggering of downstream pathways. Verveer et al. have visualized phosphorylated ErbB1 in MCF7 cells with fluoresence lifetime imaging microscopy in conjunction with fluorescence resonance energy transfer, which was used to monitor the binding of an antibody to phosphotyrosine. Focal stimulation of the cell with EGF immobilized on beads resulted in a rapid propagation of receptor phosphorylation over the entire surface of the cell--even those receptors that had not been exposed to EGF. Verveer, PJ., Wouters, F.S., Reynolds, A.R., and Bastiaens, P.I.H. (2000) Quantitative imaging of lateral ErbB1 receptor signal propagation in the plasma membrane. Science 290: 1567-1570. [Abstract] [Full Text]