RT Journal Article
SR Electronic
T1 Analyzing the Staph
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP tw351
OP tw351
DO 10.1126/stke.2001.101.tw351
VO 2001
IS 101
YR 2001
UL http://stke.sciencemag.org/content/2001/101/tw351.abstract
AB Staphylococcus aureus is a common cause of infection for which there are diminishing therapeutic options. By combining two existing technologies in a new way, Ji et al. developed a library of antisense RNAs that are inducible with tetracycline, which means the respective genes can be turned on and off not just in vitro but also in infected mice. The roles played by specific genes in bacterial growth and behavior can be examined in complex ecologies, such as at the site of infection, and thereby, clinically relevant drug or vaccine targets can be identified. Y. Ji, B. Zhang, S. F. Van Horn, P. Warren, G. Woodnutt, M. K. R. Burnham, M. Rosenberg, Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293, 2266-2269 (2001). [Abstract] [Full Text]