RT Journal Article SR Electronic T1 Analysis of Protein Arginine Methylation and Protein Arginine-Methyltransferase Activity JF Science's STKE JO Sci. STKE FD American Association for the Advancement of Science SP pl1 OP pl1 DO 10.1126/stke.2001.93.pl1 VO 2001 IS 93 A1 Mowen, Kerri A. A1 David, Michael YR 2001 UL http://stke.sciencemag.org/content/2001/93/pl1.abstract AB Posttranslational modification of proteins allows cells to adapt and react quickly to their environment beyond the boundaries set forth by genetic code. Arginine methylation, a protein modification discovered almost 30 years ago, has recently experienced a renewed interest as several new arginine methyltransferases have been identified and numerous proteins were found to be regulated by methylation on arginine residues. Until recently, the detection of arginine methylation required the use of chromatography and mass-spectrometrical analysis. The following protocol provides guidelines for the straightforward identification of arginine-methylated proteins, made possible by the availability of novel, commercially available reagents.