PT - JOURNAL ARTICLE AU - Tanimura, Natsuko AU - Nagafuku, Masakazu AU - Liddicoat, Douglas R. AU - Hamaoka, Toshiyuki AU - Kosugi, Atsushi TI - Analysis of the Mobility of Signaling Molecules in Lymphocytes Using Fluorescence Photobleaching Techniques AID - 10.1126/stke.2003.185.pl10 DP - 2003 Jun 03 TA - Science's STKE PG - pl10--pl10 VI - 2003 IP - 185 4099 - http://stke.sciencemag.org/content/2003/185/pl10.short 4100 - http://stke.sciencemag.org/content/2003/185/pl10.full SO - Sci. STKE2003 Jun 03; 2003 AB - The earliest biochemical events at the plasma membrane that lead to gene activation appear to depend not only on the local concentration of signaling molecules, but also on the mobility of these molecules at the site of signaling. To elucidate the process of signal transduction after receptor engagement in the immune system, it is important to analyze the mobility of signaling molecules in living lymphocytes. Current knowledge of the changes in intracellular localization and dynamic movements of signaling molecules during lymphocyte activation is limited. Here, we describe a method for known as fluorescence recovery after photobleaching, used to measure the diffusion mobility of a signaling molecule in a T cell line after T cell receptor stimulation. This method is a useful tool in studies of spatiotemporal regulation in immunoreceptor signaling.