PT  - JOURNAL ARTICLE
AU  - Tanimura, Natsuko
AU  - Nagafuku, Masakazu
AU  - Liddicoat, Douglas R.
AU  - Hamaoka, Toshiyuki
AU  - Kosugi, Atsushi
TI  - Analysis of the Mobility of Signaling Molecules in Lymphocytes Using Fluorescence Photobleaching Techniques
AID  - 10.1126/stke.2003.185.pl10
DP  - 2003 Jun 03
TA  - Science's STKE
PG  - pl10--pl10
VI  - 2003
IP  - 185
4099  - http://stke.sciencemag.org/content/2003/185/pl10.short
4100  - http://stke.sciencemag.org/content/2003/185/pl10.full
SO  - Sci. STKE2003 Jun 03; 2003
AB  - The earliest biochemical events at the plasma membrane that lead to gene activation appear to depend not only on the local concentration of signaling molecules, but also on the mobility of these molecules at the site of signaling. To elucidate the process of signal transduction after receptor engagement in the immune system, it is important to analyze the mobility of signaling molecules in living lymphocytes. Current knowledge of the changes in intracellular localization and dynamic movements of signaling molecules during lymphocyte activation is limited. Here, we describe a method for known as fluorescence recovery after photobleaching, used to measure the diffusion mobility of a signaling molecule in a T cell line after T cell receptor stimulation. This method is a useful tool in studies of spatiotemporal regulation in immunoreceptor signaling.