RT Journal Article SR Electronic T1 Analysis of the Mobility of Signaling Molecules in Lymphocytes Using Fluorescence Photobleaching Techniques JF Science's STKE JO Sci. STKE FD American Association for the Advancement of Science SP pl10 OP pl10 DO 10.1126/stke.2003.185.pl10 VO 2003 IS 185 A1 Tanimura, Natsuko A1 Nagafuku, Masakazu A1 Liddicoat, Douglas R. A1 Hamaoka, Toshiyuki A1 Kosugi, Atsushi YR 2003 UL http://stke.sciencemag.org/content/2003/185/pl10.abstract AB The earliest biochemical events at the plasma membrane that lead to gene activation appear to depend not only on the local concentration of signaling molecules, but also on the mobility of these molecules at the site of signaling. To elucidate the process of signal transduction after receptor engagement in the immune system, it is important to analyze the mobility of signaling molecules in living lymphocytes. Current knowledge of the changes in intracellular localization and dynamic movements of signaling molecules during lymphocyte activation is limited. Here, we describe a method for known as fluorescence recovery after photobleaching, used to measure the diffusion mobility of a signaling molecule in a T cell line after T cell receptor stimulation. This method is a useful tool in studies of spatiotemporal regulation in immunoreceptor signaling.