RT Journal Article
SR Electronic
T1 Analysis of the Mobility of Signaling Molecules in Lymphocytes Using Fluorescence Photobleaching Techniques
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP pl10
OP pl10
DO 10.1126/stke.2003.185.pl10
VO 2003
IS 185
A1 Tanimura, Natsuko
A1 Nagafuku, Masakazu
A1 Liddicoat, Douglas R.
A1 Hamaoka, Toshiyuki
A1 Kosugi, Atsushi
YR 2003
UL http://stke.sciencemag.org/content/2003/185/pl10.abstract
AB The earliest biochemical events at the plasma membrane that lead to gene activation appear to depend not only on the local concentration of signaling molecules, but also on the mobility of these molecules at the site of signaling. To elucidate the process of signal transduction after receptor engagement in the immune system, it is important to analyze the mobility of signaling molecules in living lymphocytes. Current knowledge of the changes in intracellular localization and dynamic movements of signaling molecules during lymphocyte activation is limited. Here, we describe a method for known as fluorescence recovery after photobleaching, used to measure the diffusion mobility of a signaling molecule in a T cell line after T cell receptor stimulation. This method is a useful tool in studies of spatiotemporal regulation in immunoreceptor signaling.