RT Journal Article SR Electronic T1 Image-Based Screening of Signal Transduction Assays JF Science's STKE JO Sci. STKE FD American Association for the Advancement of Science SP pe14 OP pe14 DO 10.1126/stke.2003.177.pe14 VO 2003 IS 177 A1 Ramm, Peter A1 Thomas, Nick YR 2003 UL http://stke.sciencemag.org/content/2003/177/pe14.abstract AB Imaging techniques have played a vital role in signal transduction research over several decades. Recently, industrialized macro- and micro-imaging systems have found application in drug discovery laboratories, where they increase the throughput and efficiency of drug screening. Macro-imagers are used for primary screening, where they favor compound conservation (through assay miniaturization), and achieve unprecedented rates of throughput. Micro-imaging systems achieve relatively high throughput, at the same time providing sub-cellular resolution with fixed or living cells. These micro-imaging analyses were previously conducted at very low throughput and, typically, were the sole domain of the academic researcher. Although both macro and micro forms of image-based screening remain technologies in development, they have already made substantial contributions to screening programs and will continue to do so.