RT Journal Article
SR Electronic
T1 Image-Based Screening of Signal Transduction Assays
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP pe14
OP pe14
DO 10.1126/stke.2003.177.pe14
VO 2003
IS 177
A1 Ramm, Peter
A1 Thomas, Nick
YR 2003
UL http://stke.sciencemag.org/content/2003/177/pe14.abstract
AB Imaging techniques have played a vital role in signal transduction research over several decades. Recently, industrialized macro- and micro-imaging systems have found application in drug discovery laboratories, where they increase the throughput and efficiency of drug screening. Macro-imagers are used for primary screening, where they favor compound conservation (through assay miniaturization), and achieve unprecedented rates of throughput. Micro-imaging systems achieve relatively high throughput, at the same time providing sub-cellular resolution with fixed or living cells. These micro-imaging analyses were previously conducted at very low throughput and, typically, were the sole domain of the academic researcher. Although both macro and micro forms of image-based screening remain technologies in development, they have already made substantial contributions to screening programs and will continue to do so.