RT Journal Article
SR Electronic
T1 Protein Interaction in the Gaseous Phase
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP tw449
OP tw449
DO 10.1126/stke.3142005tw449
VO 2005
IS 314
YR 2005
UL http://stke.sciencemag.org/content/2005/314/tw449.abstract
AB The identification of transient or readily reversible interactions between proteins is a difficult problem that has been addressed with various methods. Ruotolo et al. have now applied mass spectrometry to the problem to exploit its advantages of sensitivity and speed. They show that the trp RNA binding attenuation protein (TRAP) maintains its 11-membered ringlike structure in the gas phase and that binding of RNA and tryptophan influences the shape and stability of the ring in a fashion consistent with its known behavior in aqueous solution. B. T. Ruotolo, K. Giles, I. Campuzano, A. M. Sandercock, R. H. Bateman, C. V. Robinson, Evidence for macromolecular protein rings in the absence of bulk water. Science 310, 1658-1661 (2005). [Abstract] [Full Text]