RT Journal Article
SR Electronic
T1 Target Identification Tool
JF Science's STKE
JO Sci. STKE
FD American Association for the Advancement of Science
SP tw210
OP tw210
DO 10.1126/stke.3902007tw210
VO 2007
IS 390
A1 Jasny, Barbara
YR 2007
UL http://stke.sciencemag.org/content/2007/390/tw210.abstract
AB A challenge for functional genomics has been to make meaningful global measurements of the interactions between transcription factors (and cofactors) and DNA. It has been difficult, especially in large genomes, to explicitly map individual binding sites and individual factor-target gene interactions. Johnson et al. (see the Perspective by Fields) have developed a combination of chromatin immunoprecipitation and ultrahigh-throughput sequencing to achieve high specificity and 50-base pair resolution. This approach was used to study regulation by neuron-restrictive silencer factor (NRSF, also known as REST, for repressor element-1 silencing transcription factor) and identify targets of key positive regulators of pancreatic neuroendocrine development. D. S. Johnson, A. Mortazavi, R. M. Myers, B. Wold, Genome-wide mapping of in vivo protein-DNA interactions. Science 316, 1497-1502 (2007). [Abstract] [Full Text] S. Fields, Site-seeing by sequencing. Science 316, 1441-1442 (2007). [Summary] [Full Text]