RT Journal Article SR Electronic T1 Using a Microfluidic Device for High-Content Analysis of Cell Signaling JF Science Signaling JO Sci. Signal. FD American Association for the Advancement of Science SP pl2 OP pl2 DO 10.1126/scisignal.275pl2 VO 2 IS 75 A1 Cheong, Raymond A1 Wang, Chiaochun Joanne A1 Levchenko, Andre YR 2009 UL http://stke.sciencemag.org/content/2/75/pl2.abstract AB Quantitative analysis and understanding of signaling networks require measurements of the location and activities of key proteins over time, at the level of single cells, in response to various perturbations. Microfluidic devices enable such analyses to be conducted in a high-throughput and in a highly controlled manner. We describe in detail how to design and use a microfluidic device to perform such information-rich experiments.