RT Journal Article
SR Electronic
T1 Using a Microfluidic Device for High-Content Analysis of Cell Signaling
JF Science Signaling
JO Sci. Signal.
FD American Association for the Advancement of Science
SP pl2
OP pl2
DO 10.1126/scisignal.275pl2
VO 2
IS 75
A1 Cheong, Raymond
A1 Wang, Chiaochun Joanne
A1 Levchenko, Andre
YR 2009
UL http://stke.sciencemag.org/content/2/75/pl2.abstract
AB Quantitative analysis and understanding of signaling networks require measurements of the location and activities of key proteins over time, at the level of single cells, in response to various perturbations. Microfluidic devices enable such analyses to be conducted in a high-throughput and in a highly controlled manner. We describe in detail how to design and use a microfluidic device to perform such information-rich experiments.