RT Journal Article
SR Electronic
T1 Targeting the Reversibly Oxidized Protein Tyrosine Phosphatase Superfamily
JF Science Signaling
JO Sci. Signal.
FD American Association for the Advancement of Science
SP pl2
OP pl2
DO 10.1126/scisignal.3137pl2
VO 3
IS 137
A1 Boivin, Benoit
A1 Yang, Ming
A1 Tonks, Nicholas K.
YR 2010
UL http://stke.sciencemag.org/content/3/137/pl2.abstract
AB Controlled production of reactive oxygen species leads to reversible oxidation of protein tyrosine phosphatases (PTPs) and has emerged as an important tier of regulation over phosphorylation-dependent signal transduction. We present a modified cysteinyl-labeling assay that detects reversible oxidation of members of each of the different PTP subclasses. Here, we describe the methods for enriching reversibly oxidized PTPs from complex protein extracts, illustrating the procedure in IMR90 fibroblasts.