PT  - JOURNAL ARTICLE
AU  - Ray, L. Bryan
TI  - Mass and Flow
AID  - 10.1126/scisignal.4172ec130
DP  - 2011 May 10
TA  - Science Signaling
PG  - ec130--ec130
VI  - 4
IP  - 172
4099  - http://stke.sciencemag.org/content/4/172/ec130.short
4100  - http://stke.sciencemag.org/content/4/172/ec130.full
SO  - Sci. Signal.2011 May 10; 4
AB  - Measurement of multiple parameters on individual cells in conventional flow cytometry is limited because of spectral overlap of the fluorophore markers that are detected. Bendall et al. (see the Perspective by Benoist and Hacohen) describe a technique in which more than 30 measurements can be made through the use of distinct elemental isotopes detected by mass spectrometry. This technique allows the analysis of hundreds of cells per second. Each cell is vaporized at 5500 degrees kelvin, and the markers are monitored by inductively coupled plasma–mass spectrometry (ICP-MS). The technique was used to analyze the signaling properties of various cell types in the human hematopoietic system but should also be applicable to many other systems. S. C. Bendall, E. F. Simonds, P. Qiu, E.-a. D. Amir, P. O. Krutzik, R. Finck, R. V. Bruggner, R. Melamed, A. Trejo, O. I. Ornatsky, R. S. Balderas, S. K. Plevritis, K. Sachs, D. Pe’er, S. D. Tanner, G. P. Nolan, Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science 332, 687–696 (2011). [Abstract] [Full Text] C. Benoist, N. Hacohen, Flow cytometry, amped up. Science 332, 677–678 (2011). [Abstract] [Full Text]