PT  - JOURNAL ARTICLE
AU  - Hurtley, Stella M.
TI  - Reconstituting the Right Stuff for Division
AID  - 10.1126/scisignal.aaa0462
DP  - 2014 Oct 14
TA  - Science Signaling
PG  - ec287--ec287
VI  - 7
IP  - 347
4099  - http://stke.sciencemag.org/content/7/347/ec287.short
4100  - http://stke.sciencemag.org/content/7/347/ec287.full
SO  - Sci. Signal.2014 Oct 14; 7
AB  - Cytokinesis, when two daughter cells are physically separated from one another, is the final stage of cell division. How dividing cells assemble a cleavage furrow ready for cytokinesis has long interested cell biologists. A major stumbling block to probing the underlying mechanisms has been the lack of a cell-free and fully controllable experimental system. Now, Nguyen et al. have reconstituted cytokinesis organization outside living cells, using a system derived from frog eggs. In the cell-free system, the cell cycle state is “frozen,” and the spatial scale is unusually large. The authors examined the biophysics involved in signaling during cytokinesis over many minutes and many micrometers using powerful imaging techniques. P. A. Nguyen, A. C. Groen, M. Loose, K. Ishihara, M. Wühr, C. M. Field, T. J. Mitchison, Spatial organization of cytokinesis signaling reconstituted in a cell-free system. Science 346, 244–247 (2014). [Abstract] [Full Text]