RT Journal Article
SR Electronic
T1 Real-time dynamics of peptide ligand–dependent receptor complex formation in planta
JF Science Signaling
JO Sci. Signal.
FD American Association for the Advancement of Science
SP ra76
OP ra76
DO 10.1126/scisignal.aab0598
VO 8
IS 388
A1 Somssich, Marc
A1 Ma, Qijun
A1 Weidtkamp-Peters, Stefanie
A1 Stahl, Yvonne
A1 Felekyan, Suren
A1 Bleckmann, Andrea
A1 Seidel, Claus A. M.
A1 Simon, Rüdiger
YR 2015
UL http://stke.sciencemag.org/content/8/388/ra76.abstract
AB The CLAVATA (CLV) and flagellin (flg) signaling pathways act through peptide ligands and closely related plasma membrane–localized receptor-like kinases (RLKs). The plant peptide CLV3 regulates stem cell homeostasis, whereas the bacterial flg22 peptide elicits defense responses. We applied multiparameter fluorescence imaging spectroscopy (MFIS) to characterize the dynamics of RLK complexes in the presence of ligand in living plant cells expressing receptor proteins fused to fluorescent proteins. We found that the CLV and flg pathways represent two different principles of signal transduction: flg22 first triggered RLK heterodimerization and later assembly into larger complexes through homomerization. In contrast, CLV receptor complexes were preformed, and ligand binding stimulated their clustering. This different behavior likely reflects the nature of these signaling pathways. Pathogen-triggered flg signaling impedes plant growth and development; therefore, receptor complexes are formed only in the presence of ligand. In contrast, CLV3-dependent stem cell homeostasis continuously requires active signaling, and preformation of receptor complexes may facilitate this task.