Table 2

Computationally predicted kinase specificity–determining residues. Correlation values and peptide-kinase distance measurements are defined in Materials and Methods.

Residue
(PKA
numbering)
CorrelationDistance
from
peptide (Å)
Representative
kinase
Amino
acid
SpecificityReference
1700.1955.3Tpk1Glu−2 Arg(27)
Sch9Glu−5 Arg(29), (70)
Yak1Glu−3 ArgThis study
1690.1867.2Psk2Asp−5 Basic(30)
Cmk1Pro−5 Aliphatic(18), (30)
1970.1769.5Cdc28(p)Thr+3 Basic(71)
1290.1726.4Tpk1Phe−3 Arg(27)
Atg1Thr−3 AliphaticThis study
1270.1577.4Tpk1Glu−3 Arg(27)
1990.1455.7Tpk1Cys0 SerThis study
2460.1348.4Gcn2Arg−2 AcidicThis study
2050.1228.4Kss1Arg+1 Pro(54)
Ipl1Leu+1 Hydrophobic(27), (29), (72)
2370.1209.9Yak1Leu−2 ProThis study
2030.1086.1Yck1Arg−3 pSer/pThr(73)
Mps1Asn−2 AcidicThis study
Gin4His−2 SerThis study