Table 3

Proteins phosphorylated by Prk1 in vitro. Proteins functionally associated with actin rearrangement or endocytosis are Bem2, Ede1, Las17, Sac3, Sla2, Syp1, and Yap1801. Ub, ubiquitin; RhoGAP, Rho guanosine triphosphatase–activating protein; SCF, Skp1-Cullin-F-box; UPR, unfolded protein response; WASP, Wiscott-Aldrich syndrome protein.

ORFProtein nameFunction
YBL047CEde1Key endocytic protein; binds plasma
membrane in a Ub-dependent manner
YCR030CSyp1Overexpression suppresses a pfy1
(profilin) null mutation
YDL001WRmd1Cytoplasmic protein required for sporulation
YDR159WSac3Nuclear pore–associated protein; suppressor
of actin mutations
YER155CBem2RhoGAP involved in the control of
cytoskeleton and morphogenesis
YGR094WVas1Mitochondrial and cytoplasmic valyl-tRNA
YHL030WEcm29Major component of the proteasome
YHR161CYap1801Protein involved in clathrin cage assembly;
binds clathrin and Pan1
YHR098CSfb3Involved in sorting Pma1 into COPII vesicles
YJL090CDpb11Subunit of DNA polymerase II epsilon
YJL129CTrk1Component of the Trk1-Trk2 potassium
transport system
YJL184WGon7Component of the EKC/KEOPS complex
YJR137CEcm17Sulfite reductase beta subunit involved
in amino acid biosynthesis
YML088WUfo1F-box protein subunit of the SCF E3 ubiquitin
ligase complex
YML103CNup188Subunit of the nuclear pore complex
YNL243WSla2Transmembrane protein that links actin
to clathrin and endocytosis
YNL287WSec21Gamma subunit of coatomer complex
YOR093CYor093cFunction unknown; deletion causes sensitivity
to UPR-inducing agents
YOR181WLas17Actin assembly factor; homolog of
human WASP
YPL131WRpl5Component of the large 60S ribosomal
YPR160WGph1Glycogen phosphorylase