Table 2 Differential phosphorylation of select peptides in LPS-stimulated monocytes.
Phosphoprotein*Kinase targetRelative change P value§
IRAK1Thr2093.20.01
IRAK1Ser3761.10.01
IRAK1Ser5680.20.01
IRAK1Thr3871.00.01
IRAK1Thr1001.30.01
IKKγSer432.60.01
NF-κB (p100)Ser8703.50.06
NF-κB (p105)Ser33718.40.02
NF-κB (p65)Ser27611.40.01
NF-κB (p65)Ser3114.30.01
IRF-3Ser3967.70.04
FADDSer19410.60.08
Caspase 8Ser3473.40.01

*Common name for substrate protein with a peptide for a phosphorylation site on the array.  †Target amino acid on the substrate protein.  ‡Relative change for LPS samples was calculated by comparing the background-corrected and normalized signal values of these samples to the media control.  §P values reported by GeneSpring software for normalized phosphorylation signals. Each P value represents confidence level on reproducibility of a signal and is evaluated by one-sample t test, which compares normalized signals (n = 18) for each peptide to a baseline value of 1.