Table 2 Contractile properties of TDAs isolated from Cre/Panx1WT, Cre/Panx1Fl, Cre+/Panx1WT, or Cre+/Panx1F1 mice.

The EC50 and EMAX are calculated from the data presented in Fig. 2 (C to F). EC50 represents the concentration needed to produce 50% of the maximum effect (EMAX). EMAX is expressed as the percentage of maximal diameter. Data are presented as means ± SEM. *P < 0.05, #P < 0.07 compared to Cre/Panx1WT using a Kruskal-Wallis test (n = 4 to 16).

Phenylephrine
Cre/Panx1WTCre/Panx1FLCre+/Panx1WTCre+/Panx1FL
EC50 (μM)0.66 ± 0.130.64 ± 0.371.79 ± 1.137.84 ± 5.60
EMAX51.8 ± 2.6646.3 ± 5.9453.5 ± 4.9169.1 ± 3.06*
Noradrenaline
Cre/Panx1WTCre/Panx1FLCre+/Panx1WTCre+/Panx1FL
EC50 (μM)6.28 ± 1.4510.3 ± 1.36.78 ± 3.2050.5 ± 24.8
EMAX41.4 ± 5.1045.6 ± 5.4048.2 ± 1.8256.4 ± 5.61#
Serotonin
Cre/Panx1WTCre/Panx1FLCre+/Panx1WTCre+/Panx1FL
EC50 (nM)23.8 ± 5.0765.2 ± 40.940.7 ± 12.214.2 ± 5.53
EMAX36.1 ± 2.6830.4 ± 1.7840.1 ± 4.5938.2 ± 5.60
Endothelin-1
Cre/Panx1WTCre/Panx1FLCre+/Panx1WTCre+/Panx1FL
EC50 (nM)18.4 ± 10.93.77 ± 0.565.64 ± 2.9430.1 ± 14.6
EMAX41.8 ± 4.9542.2 ± 6.5042.5 ± 4.8238.9 ± 6.90