The top cell cycle–related proteins that were pulled down with Halo-tagged FADD in HEK293T cells. The cutoff that determined FADD-interacting proteins was a spectral count (SpC) of 5 or greater and a control SpC of 0. NSAF, normalized spectral abundance factor.
| Identified proteins | Gene symbol | Molecular weight (kD) | FADD peptide SpC | Control peptide SpC | FADD NSAF | Control NSAF |
| FADD | FADD | 23 | 555 | 0 | 0.104876887 | 0 |
| CK1α | CSNK1A1 | 39 | 20 | 0 | 0.002228846 | 0 |
| Polo-like kinase 1 | PLK1 | 68 | 5 | 0 | 0.000319577 | 0 |
| Aurora kinase B | AURKB | 39 | 6 | 0 | 0.000668654 | 0 |
| Cell division cycle 20 | CDC20 | 55 | 17 | 0 | 0.001343386 | 0 |
| Centrosomal protein 170 | CEP170 | 175 | 11 | 0 | 0.000273193 | 0 |
| Structural maintenance of chromosomes 1A | SMC1A | 143 | 13 | 0 | 0.000395114 | 0 |
| Non-SMC condensing II complex, subunit D3 | NCAPD3 | 169 | 19 | 0 | 0.000166099 | 0 |
| Non-SMC condensing II complex, subunit G2 | NCAPG2 | 131 | 18 | 0 | 0.000597195 | 0 |
| Non-SMC condensing II complex, subunit D2 | NCAPD2 | 157 | 6 | 0 | 0.000166099 | 0 |
| Dynactin 2 | DCTN2 | 44 | 5 | 0 | 0.000493892 | 0 |
| Cell division cycle 73 | CDC73 | 61 | 7 | 0 | 0.0004875 | 0 |
| Fanconi anemia complementation group D2 | FANCD2 | 165 | 15 | 0 | 0.000392733 | 0 |
| Fanconi anemia complementation group I | FANCI | 149 | 36 | 0 | 0.001050101 | 0 |
| Cytoskeleton-associated protein 5 | CKAP5 | 226 | 7 | 0 | 0.000134618 | 0 |