Table 1 FADD interacts with proteins involved in the G2/M transition.

The top cell cycle–related proteins that were pulled down with Halo-tagged FADD in HEK293T cells. The cutoff that determined FADD-interacting proteins was a spectral count (SpC) of 5 or greater and a control SpC of 0. NSAF, normalized spectral abundance factor.

Identified proteinsGene
symbol
Molecular
weight (kD)
FADD
peptide SpC
Control
peptide SpC
FADD NSAFControl
NSAF
FADDFADD2355500.1048768870
CK1αCSNK1A1392000.0022288460
Polo-like kinase 1PLK168500.0003195770
Aurora kinase BAURKB39600.0006686540
Cell division cycle 20CDC20551700.0013433860
Centrosomal protein 170CEP1701751100.0002731930
Structural maintenance of chromosomes 1ASMC1A1431300.0003951140
Non-SMC condensing II complex, subunit D3NCAPD31691900.0001660990
Non-SMC condensing II complex, subunit G2NCAPG21311800.0005971950
Non-SMC condensing II complex, subunit D2NCAPD2157600.0001660990
Dynactin 2DCTN244500.0004938920
Cell division cycle 73CDC7361700.00048750
Fanconi anemia complementation group D2FANCD21651500.0003927330
Fanconi anemia complementation group IFANCI1493600.0010501010
Cytoskeleton-associated protein 5CKAP5226700.0001346180