Table 1 Effect of probenecid and 10Panx1 on TDA constriction in response to phenylephrine, noradrenaline, serotonin, or endothelin-1.

EC50 and EMAX were calculated using the cumulative concentration response curves shown in Fig. 1 (A to D). EC50 represents the concentration needed to produce 50% of the maximum effect (EMAX). EMAX is expressed as the percentage of maximal diameter. Data are presented as means ± SEM. *P < 0.05 compared to control using a Kruskal-Wallis test (n = 5 to 7).

PhenylephrineNoradrenaline
ControlProbenecid10Panx1ControlProbenecid10Panx1
EC50 (μM)1.21 ± 0.272.71 ± 1.362.38 ± 0.530.56 ± 0.251.30 ± 0.651.61 ± 0.49
EMAX41.1 ± 2.2172.6 ± 3.51*60.6 ± 5.80*33.3 ± 1.0474.9 ± 3.04*73.4 ± 10.6*
SerotoninEndothelin-1
ControlProbenecid10Panx1ControlProbenecid10Panx1
EC50 (nM)50.8 ± 18.983.3 ± 8.4247.9 ± 23.14.57 ± 0.8812.8 ± 4.056.53 ± 1.36
EMAX34.8 ± 2.6639.5 ± 3.2930.9 ± 4.9241.9 ± 4.8234.6 ± 4.5041.5 ± 3.91