Table 5 Effect of transfection of TDAs with Panx1WT or Panx1YLK>AAA on phenylephrine-induced constriction.

The EC50 and EMAX are calculated from the data presented in Fig. 4 (E and F). EC50 represents the concentration needed to produce 50% of the maximum effect (EMAX). EMAX is expressed as the percentage of maximal diameter. Data are presented as means ± SEM. *P < 0.05 in comparison to no tamoxifen, and #P < 0.05 in comparison to Cre+/Panx1FL + Panx1WT using a Kruskal-Wallis test.

Phenylephrine
Cre+/Panx1FL No tamoxifenCre+/Panx1FL With tamoxifenCre+/Panx1FL + Panx1WTCre+/Panx1FL + Panx1YLK>AAA
EC50 (μM)0.64 ± 0.231.13 ± 0.770.45 ± 0.330.63 ± 0.53
EMAX32.9 ± 4.8557.5 ± 9.20*#34.2 ± 6.1556.9 ± 5.84*#