Table 3 Multivariate survival analysis by Cox proportional hazard models.
AdjustedAdjusted and corrected for overoptimism
HR (95% CI)PHR (95% CI)
Overall survival (n = 198)*
CHEK1 expression
  <Median (reference)1.001.00
  >Median1.74 (1.19–2.56)0.0041.58 (1.15–2.17)
Age1.00 (0.99–1.02)0.26
Year of diagnosis0.96 (0.91–1.02)0.26
White blood cells (g/liter)
  0.7–6.5 (reference)1.00
  6.5–30.01.40 (0.81–2.42)0.23
  30.0–73.02.09 (1.22–3.59)0.008
  73.0–3561.91 (1.11–3.31)0.02
Sex
  Men (reference)1.00
  Women1.06 (0.72–1.55)0.77
Cytogenetic risk group
  Favorable (reference)1.00
  Intermediate3.16 (1.50–6.65)0.002
  Adverse7.51 (3.35–16.84)<0.001
Event-free survival (n = 198)
CHEK1 expression
  <Median (reference)1.001.00
  >Median1.74 (1.21–2.52)0.0031.58 (1.17–2.14)
Age1.01 (0.99–1.03)0.19
Year of diagnosis0.99 (0.94–1.05)0.83
White blood cells (g/liter)
  0.7–6.5 (reference)1.00
  6.5–30.01.40 (0.83–2.37)0.21
  30.0–73.02.17 (1.28–3.69)0.004
  73.0–3561.86 (1.10–3.15)0.02
Sex
  Men (reference)1.00
  Women0.90 (0.62–1.31)0.59
Cytogenetic risk group
  Favorable (reference)1.00
  Intermediate2.80 (1.43–5.48)0.003
  Adverse6.27 (2.97–13.24)<0.001
Relapse-free survival (n = 162)
CHEK1 expression
  <Median (reference)1.00
  >Median2.10 (1.35–3.29)0.0011.74 (1.25–2.43)
Age (years)
  18–371.00
  37–481.19 (0.64–2.18)0.58
  48–570.82 (0.45–1.49)0.52
  57–651.48 (0.83–2.63)0.18
Year of diagnosis0.97 (0.91–1.05)0.46
White blood cells (g/liter)
  0.7–6.5 (reference)1.00
  6.5–30.01.73 (0.90–3.32)0.10
  30.0–73.02.62 (1.34–5.12)0.005
  73.0–3562.40 (1.28–4.50)0.007
Sex
  Men1.00
  Women0.91 (0.58–1.43)0.67
Cytogenetic risk group
  Favorable (reference)1.00
  Intermediate2.83 (1.37–5.82)0.002
  Adverse6.47 (2.71–15.46)<0.001

*HRs have been corrected for overoptimism, applying an internal validation procedure based on cross-validation. Slope index was 0.82.

†HRs have been corrected for overoptimism, applying an internal validation procedure based on cross-validation. Slope index was 0.82.

‡HRs have been corrected for overoptimism, applying an internal validation procedure based on cross-validation. Slope index was 0.75.