Determination of the parameters p and q derived from the quantitative analysis of the blinking number distributions for the calibration proteins CD86_mEos2 and CTLA-4_mEos2. The average localization precision calculated with a NeNA is shown. Data are means ± SEM.
p | q | Ncells | Nanalyzed clusters | Average localization precision (nm) | |
CD86_mEos2 | 0.32 | — | 9 | 504 | 15.1 ± 0.4 |
CTLA-4_mEos2 | — | 0.29 | 10 | 844 | 16.1 ± 1.0 |