Table 1 Values for the calibration proteins used for quantitative SMLM.

Determination of the parameters p and q derived from the quantitative analysis of the blinking number distributions for the calibration proteins CD86_mEos2 and CTLA-4_mEos2. The average localization precision calculated with a NeNA is shown. Data are means ± SEM.

pqNcellsNanalyzed clustersAverage localization precision (nm)
CD86_mEos20.32950415.1 ± 0.4
CTLA-4_mEos20.291084416.1 ± 1.0