Table 1 Mutation data for the cell lines.

Mutation data for selected critical genes for the nine neuroblastoma cell lines used in this study. MNA, MYCN-amplified; HomZ., homozygote; mut, mutant; amp, amplification; ex, exon; expr., expression.

Cell lineGenomic profile*ALK status (amp, mut ex23–25)MYCN statusTP53 status mut ex5–9NRAS statusNF1 expr.
CLB-BARMNAAmp. whole ALK exc. part
of i3 (nonamp). Note, exome
sequencing shows del ex4–11
MYCN-amplifiedNo mut in TP53No mut in NRASHigh
CLB-GEMNAALK amp. ALK mut F1174VMYCN-amplifiedNo mut in TP53No mut in NRASHigh
CLB-GAR11q-del2p gain with break
within ALK intron 1.
Mutation R1275Q
Not MYCN-amplifiedNo mut in TP53No mut in NRASHigh
KellyMNA + 11q-delNo amplification. 2p-gain.
Mutation F1174L
MYCN-amplifiedHomZ. Mut P177TNo mut in NRASHigh
SK-N-DZMNANo mutation.
No amplification
MYCN-amplifiedNo mut in TP53No mut in NRASIntermediate
CLB-PEMNA + 11q-del2p gain. No mutation.
No amplification
MYCN-amplifiedHomZ. mut C176FNo mut in NRASHigh
IMR32MNAAmp. of ALK ex3–4 only.
No mutation
MYCN-amplifiedNo mut in TP53No mut in NRASHigh
SK-N-AS11q-delNo mutation.
No amplification
Not MYCN-amplifiedNo mut in TP53Mut in NRAS: Q61KLow
SK-N-BEMNA2p gain. No mutation.
No amplification
MYCN-amplifiedHomZ. mut C145FNo mut in NRASLow

*Genomic profile as defined in (74).

†Genomic profile as defined in (75).