Table 2 Structural parameters for FixL and FixL-FixJ complexes determined by SEC-SAXS experiments at BL45XU in SPring-8.
SampleGuinier Rg
(Å)
Dmax (Å)Porod
volume Vp
3)
Molecular
mass from
Vp (kDa)
Met FixL49.7 ± 0.1163 ± 4224,220 ±
3,061
140.1 ± 1.9
Cyanomet
FixL*
48.4 ± 0.2158 ± 3218,075 ±
3,693
136.3 ± 2.3
Truncated
FixL
34.0121100,79063.0
FixJ22.3 ± 0.266 ± 239,230 ±
1,115
24.5 ± 0.7
Met FixL-FixJ
complex
53.1 ± 0.1160 ± 5296,970 ±
2,478
185.4 ± 1.5
Cyanomet
FixL-FixJ
complex
52.1 ± 0.1163 ± 3273,820 ±
2,253
171.1 ± 1.4
Met
FixL-FixJN
complex
48.9 ± 0.1170 ± 3245,753 ±
2,306
153.6 ± 1.4

*The data collection was performed using KCN-containing buffer [40 mM tris-HCl (pH 8.0), 10% (w/v) glycerol, 5 mM potassium cyanide, and 5 mM MgCl2].

The parameters for truncated FixL were obtained by one measurement. Other samples were analyzed from multiple measurements (n > 3).