Table 2 Effect of adenosine on the chemotaxis of HD and HNSCC CD8+ T cells.

Activated CD8+ T cells from HD and HNSCC patients were exposed to a gradient of either CXCL12 or CXCL12 and adenosine (ADO), and the indicated values were measured. Results are presented as means ± SEM for all measured values. Y-COM, center of mass along the y axis, along the chemokine gradient; X-COM, center of mass along the x axis, perpendicular to the chemokine gradient; FMIy, forward migration index in the direction of the y axis (represents the efficiency of forward migration toward the chemokine gradient); FMIx, forward migration index in the direction of the x axis (represents the efficiency of migration perpendicular to the chemokine gradient respectively).

ParameterCXCL12CXCL12 + ADOP value (CXCL12 versus
CXCL12 + ADO)
HD (n = 5)
X-COM (μm)5.660 ± 4.7391.586 ± 5.2820.610
Y-COM (μm)43.279 ± 9.221*33.366 ± 10.4240.134
FMIx0.016 ± 0.019−0.001 ± 0.0150.523
FMIy0.141 ± 0.0310.109 ± 0.029§0.001
Directness0.203 ± 0.0240.202 ± 0.0170.925
Velocity (μm/s)0.210 ± 0.0210.182 ± 0.0210.478
Accumulated distance (μm)315.462 ± 31.352272.336 ± 30.9090.475
Euclidean distance (μm)62.122 ± 6.27756.081 ± 10.1040.526
HNSCC (n = 6)
X-COM (μm)−7.125 ± 9.5963.943 ± 11.9830.629
Y-COM (μm)46.773 ± 18.178ǁ¶4.281 ± 4.829#0.029
FMIx−0.030 ± 0.0230.024 ± 0.0360.404
FMIy0.191 ± 0.039**0.005 ± 0.020††<0.001
Directness0.256 ± 0.0400.188 ± 0.0170.080
Velocity (μm/s)0.146 ± 0.0420.139 ± 0.0400.495
Accumulated distance (μm)211.297 ± 62.163206.839 ± 59.6200.725
Euclidean distance (μm)38.126 ± 8.52438.145 ± 10.9530.438

*P = 0.002 versus X-COM (in HD).

P = 0.0876 versus X-COM (in HD).

P = 0.008 versus FMIx (in HD).

§P = 0.046 versus FMIx (in HD).

ǁP = 0.031 versus X-COM (in HNSCC).

¶Statistical significance is measured by Wilcoxon signed-rank test; all other P values are measured by paired Student’s t test.

#P = 0.970 versus X-COM (in HNSCC).

**P = 0.011 versus FMIx (in HNSCC).

††P = 0.610 versus FMIx (in HNSCC).