Table 2 Potency (pEC50) and maximal response (Emax) of β-arrestin2 recruitment with GRK overexpression (OE) or knockdown (KD).
MORBRETβ-Gal
pEC50Emax
(% of mock DAMGO)
pEC50Emax
(% of mock or SCR)
MockDAMGO6.50 ± 0.021005.72 ± 0.02100
Morphine5.95 ± 0.0525.72 ± 2.106.51 ± 0.38100
GRK2-WT
OE
DAMGO8.03 ± 0.04^176.43 ± 8.43^5.81 ± 0.01128.98 ± 17.03
Morphine7.33 ± 0.05^146.06 ± 6.18^6.16 ± 0.26183.99 ± 28.26
GRK2-DN
OE
DAMGO6.41 ± 0.0362.23 ± 3.18^
Morphine6.55 ± 0.0212.56 ± 1.66
GRK3-WT
OE
DAMGONANA6.51 ± 0.03157.94 ± 18.66
MorphineNANA6.05 ± 0.14144.58 ± 40.65
SCRDAMGONANA5.71 ± 0.13100
MorphineNANA
GRK2 KDDAMGONANA6.09 ± 0.1272.39 ± 4.35
MorphineNANA
GRK3 KDDAMGONANA6.14 ± 0.1585.64 ± 2.51
MorphineNANA

^Significance compared to WT (P < 0.01) by two-way ANOVA with Dunnett’s multiple comparison test.