Table 1 Potency (pEC50) and maximal response (Emax) of β-arrestin1/2 recruitment to WT and phosphorylation-deficient MOR mutants.

NA, not available.

MORβ-Arrestin1β-Arrestin2
BRETβ-GalBRETβ-Gal
pEC50Emax
(% of WT
DAMGO)
pEC50Emax
(% of WT
DAMGO)
pEC50Emax
(% of WT
DAMGO)
pEC50Emax
(% of WT
DAMGO)
WTDAMGO6.05 ± 0.061005.82 ± 0.071006.28 ± 0.041005.94 ± 0.03100
Morphine6.12 ± 0.0533.57 ± 2.18
TSST-4ADAMGO6.10 ± 0.0691.59 ± 1.565.86 ± 0.0536.33 ± 6.79^6.36 ± 0.0294.08 ± 3.765.58 ± 0.0556.73 ± 7.61^
Morphine5.85 ± 0.1429.21 ± 4.11
S375ADAMGO5.89 ± 0.0736.20 ± 6.27^6.71 ± 0.1332.20 ± 5.73^6.30 ± 0.0171.73 ± 6.47^6.08 ± 0.1849.36 ± 16.95^
Morphine6.37 ± 0.1417.31 ± 0.90^
STANT-3ADAMGONANA6.20 ± 0.0656.26 ± 1.81^NANA
MorphineNANA6.40 ± 0.0814.76 ± 1.66^NANA
TREHPSTANT-4ADAMGO5.95 ± 0.4828.47 ± 7.18^NANA5.72 ± 0.0542.55 ± 6.31^
MorphineNANA
11S/T-ADAMGO8.27 ± 1.7423.46 ± 2.33^6.16 ± 0.0241.90 ± 5.48^6.08 ± 0.1743.73 ± 5.16^
Morphine6.29 ± 0.0815.92 ± 1.67^

^Significance compared to WT (P < 0.01) by two-way ANOVA with Dunnett’s multiple comparison test.