Table 4 Bias analysis between [35S]GTPγS binding and forskolin-stimulated cAMP accumulation for SH-SY5Y-hKOR cells.

LogR, ΔLogR, ΔΔLogR, and bias factors (10ΔΔlogRassay1-assay2) were calculated using U69,593 as the reference agonist, as described in “Data analysis and statistics” using the values stated in the figure legends of Fig. 4. Data are presented as means ± SEM from n ≥ 3 independent experiments performed in duplicate to triplicate. G, [35S]GTPγS; cAMP, forskolin-stimulated cAMP accumulation; 95% CI, 95% confidence interval.

Compound[35S]GTPγS bindingcAMPΔΔLogR (95% CI)Bias factor (G/cAMP)
LogRΔLogRLogRΔLogR
U69,5937.18 ± 0.0606.32 ± 0.30001
1.17.06 ± 0.15−0.112 ± 0.096.26 ± 0.30−0.059 ± 0.12−0.053(−0.43 to 0.32)0.9
2.16.74 ± 0.13−0.434 ± 0.086.14 ± 0.48−0.219 ± 0.06−0.215 (−0.46 to 0.03)0.6