Table 2 Analysis of bias factors between functional assays.

Using U69,593 as a reference agonist, the ΔΔLogR and bias factors (10ΔΔlogRassay1-assay2) were calculated as described in “Data analysis and statistics” (Materials and Methods) using the ΔLogR values listed in Table 1. GTP, [35S]GTPγS binding assay; βarr2, β-arrestin2 recruitment assay (EFC); cAMP, forskolin-stimulated cAMP accumulation assay; Ca2+, intracellular calcium mobilization assay; GIRK, GIRK channel activation assay; 95% CI, 95% confidence interval.

Comparison of assays1.12.1
ΔΔLogR (95% CI)Bias factorΔΔLogR (95% CI)Bias factor
GTP/βarr21.27 (1.03 to 1.52)190.98 (0.73 to 1.22)9
GTP/cAMP1.35 (1.02 to 1.69)230.89 (0.64 to 1.13)8
GTP/Ca2+1.62 (1.24 to 1.99)421.38 (0.94 to 1.82)24
GTP/GIRK−0.06 (−0.30 to 0.18)0.9−0.04 (−0.39 to 0.32)0.9
cAMP/GTP−1.35 (−1.69 to −1.02)0.04−0.89 (−1.13 to −0.64)0.1
cAMP/βarr2−0.08 (−0.46 to 0.30)0.80.09 (−0.20 to 0.38)1
cAMP/Ca2+0.26 (−0.21 to 0.74)20.49 (0.03 to 0.95)3
cAMP/GIRK−1.41 (−1.88 to −0.94)0.04−0.92 (−1.34 to -0.50)0.1
Ca2+/GTP−1.62 (−1.99 to −1.24)0.02−1.38 (−1.82 to −0.94)0.04
Ca2+/βarr2−0.35 (−0.77 to 0.08)0.5−0.40 (−0.91 to 0.10)0.4
Ca2+/cAMP−0.26 (−0.74 to 0.21)0.5−0.49 (−0.95 to -0.03)0.3
Ca2+/GIRK−1.68 (−2.21 to −1.14)0.02−1.41 (−2.12 to −0.71).0.03
GIRK/GTP0.06 (−0.18 to 0.03)1.10.04 (−0.32 to 0.39)1
GIRK/βarr21.33 (1.04 to 1.62)211.01 (0.57 to 1.46)10
GIRK/cAMP1.41 (0.94 to 1.88)260.92 (0.50 to 1.34)8
GIRK/Ca2+1.68 (1.14 to 2.21)481.41 (0.71 to 2.12)26