Table 1 Merlin-proximal proteins.

The proteins that met the selection criteria from proximity biotinylation assays with Merlin-BirAR118G are arranged by the average number of MS peptide reads normalized to protein molecular weight, along with the number of peptides from these proteins identified in all other samples. n = 3 independent experiments. Merlin and known Merlin-binding proteins Angiomotin (Amot), angiomotin-like protein 1 (Amotl1), Erbin (Erbb2ip), and moesin (Msn) are highlighted in bold.

GeneDescriptionMWCtrlWTBMFHAR6N
Nf2Neurofibromin 2 (Merlin)69,7764.7187.381.6220.8241.7124.7
Rai14Retinoic acid induced 14108,85211.28019.249.36126.3
Epb41l2Erythrocyte membrane protein band 4.1-like 2109,94010.948.718.58154.719.4
UtrnUtrophin392,70613.9101.512.1137.810023.7
Mllt4Afadin206,4991.948.35.948.129.36.8
Amotl1Angiomotin-like protein 1107,9500.624.49.13.626.98.5
Ruvbl1RuvB-like AAA ATPase 150,2142.211.21914.614
Kank2KN motif and ankyrin repeat domains 290,2454.419.63.521.216.69.1
Epb41l3Erythrocyte membrane protein band 4.1-like 3103,3382.820.35.433.329.47.9
Epb41l1Erythrocyte membrane protein band 4.1-like 198,315019.37.318.714.62.2
Tjp1ZO-1, tight junction protein 1194,7423.437.36.521.4265.1
Tp53bp2ASPP2, tumor protein p53 binding protein 2125,3011.821.50.413.317.82.9
Rassf8Ras association domain family member 848,10308.20.85.89.91.7
VclVinculin116,7171.217.11.26.516.98
PrunePrune exopolyphosphatase50,239071.72.46.42.9
MpripMyosin phosphatase Rho-interacting protein116,4082.516.22.424.422.85
Erc1ELKS/RAB6-interacting/CAST family member 1128,3313.117.81.714.22610.3
AmotAngiomotin120,915016.22014.44.3
Phldb2Pleckstrin homology-like domain, family B member 2141,4861.318.93.412.2148.1
Dlg5Discs, large homolog 5214,386028.50526.96.4
Erbb2ipErbb2-interacting protein157,248019.33.423.614.90.5
Myh10Myosin heavy chain 10, nonmuscle228,9967.525.643.49.921.135
LppLipoma-preferred partner65,89117.12.16.111.76.6
Cobll1Cordon-bleu WH2 repeat protein-like 1137,3820.614.80.423.78.91.6
Stk38Serine/threonine kinase 3854,1740.35.71.14.54.43.9
MsnMoesin54,17405.73.57.915.26.5
Cep55Centrosomal protein of 55 kDa53,93005.30.201.80
Actn1Actinin, a1103,068010.14.30.4125.2
ScribScribbled planar cell polarity protein174,0590.616.42.515.616.73.8
Dlg1Discs, large homolog 1100,12009.31.58.612.80
Phldb1Pleckstrin homology-like domain, family B, member 1150,0700.613.81.218.311.31.4
Shroom4Shroom family member 4163,2381.814.81.219.38.62.1
Ptpn13Protein tyrosine phosphatase, type 13270,3341.324.47.823.8225.1
MpdzMultiple PDZ domain protein218,711019.20.72.419.62.7
Tnks1bp1Tankyrase 1 binding protein 1, 182 kDa181,8251.514.92.631.319.87
Tjp2Tight junction protein 2131,2800.310.61.243.93.2
Phactr4Phosphatase and actin regulator 476,63205.80.66.140.5
Fam129bFamily with sequence similarity 129, member B84,819063.72.662.5
Ctnnd1Catenin (cadherin-associated protein), d1104,92507.31.65.85.31.4
Peak1Pseudopodium-enriched atypical kinase 1191,0970.612.75.617.413.14.8
Tln2Talin 2253,6210.315.107.68.30.5
TriobpTRIO and F-actin binding protein223,3680.913.2216.411.61.6
Sipa1Signal-induced proliferation-associated 1112,0660.660.44.583.5
Sipa1l1Signal-induced proliferation-associated 1-like 1197,03109.91.94.160.9
Magi3Membrane associated guanylate kinase 3161,67206.72.303.50
Ehbp1l1EH domain binding protein 1-like 1184,83407.4019.97.81.6
CgnCingulin136,44705.3002.30
Rrbp1Ribosome binding protein 1172,8790.65.711.72.210.15.8
Magi1Membrane associated guanylate kinase 1161,97405.33.81.620
Atg2bAutophagy-related 2B231,39907.11.42.43.55.5
Itsn1Intersectin 1194,29705.608.55.10
SpegSPEG complex locus354,3430.98.719.111.213.41
TrioTrio Rho guanine nucleotide exchange factor347,8610.66.31.49.650.7