Table 1 Blood metabolite analysis of MBKO mice.

Sera were collected from mice (6 months old; n = 4 to 8 mice per group) fed ad libitum or fasted for 24 hours. Results are expressed as the means ± SEM. CK, creatine kinase; FFA, free fatty acid; TG, triglyceride.

FedFasted
Fl/FlMBKOFl/FlMBKO
Glucose (mg/dl)128.4 ± 8.2140.7 ± 6.892.1 ± 3.8b119.9 ± 10.2*
Insulin (ng/ml)0.63 ± 0.080.88 ± 0.080.35 ± 0.06a0.74 ± 0.09*
TG (mM)1.03 ± 0.081.51 ± 0.20*3.46 ± 0.54c5.90 ± 0.71*, c
FFA (mM)0.70 ± 0.081.04 ± 0.161.09 ± 0.251.63 ± 0.14a
BDNF (ng/ml)5.49 ± 2.833.61 ± 0.9342.46 ± 5.42c14.69 ± 5.64*
Alanine (nM)
CK activity (nmol/min per
milliliter)
0.33 ± 0.02
39.36 ± 20.27
0.30 ± 0.02
53.19 ± 9.74
0.67 ± 0.07b
37.10 ± 18.32
0.39 ± 0.01**,b
98.47 ± 1.05**,b

*P < 0.05 compared to Fl/Fl mice of the same treatment group, Student’s t test.

**P < 0.01 compared to Fl/Fl mice of the same treatment group, Student’s t test.

***P < 0.001 compared to Fl/Fl mice of the same treatment group, Student’s t test.

aP < 0.05 compared to the same genotype, Student’s t test.

bP < 0.01 compared to the same genotype, Student’s t test.

cP < 0.001 compared to the same genotype, Student’s t test.